Read More About:
byAlma Abell
CD31 is considered to be a glycoprotein that is expressed in platelets and on endothelial cells. It is involved in cell adhesion and cell signaling. It can be helpful in a variety of research needs, but learning to use it effectively is imperative.
More Information
The CD31 antibody has a clone called SP38, and the immunogen is the synthetic peptide that corresponds to the C-terminus of the mouse protein of the same name. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of between 100 and 130 kDa.
Applications
The CD31 antibody can be used with Immunohistochemistry applications. To prepare the specimen, use a paraffin-embedded or Formalin-fixed tissue sample. Deparaffinized slides are essential and can be completed using xylene, an alternative to xylene, or graded alcohols.
When you choose to work with the concentrate formula, you will need to dilute the antibody using a ratio of 1:50 in an Antibody Diluent. You can also find a pre-diluted formulation that will work for these ratios. Dilutions are estimates, however, so it is essential that you follow your methods and protocols.
To retrieve the antigen, you should boil the tissue section in a Citrate buffer with a pH of 6.0. This should be done for ten minutes and then allowed to cool to room temperature for 20 minutes. You will also need to incubate it for ten minutes while at room temperature.
You should wash the slides between steps and can rinse them using a PBS/0.05 percent Tween solution.
The positive control is the tonsil with cellular localization occurring in the membrane.
The CD31 antibody can help with signaling cells and much more. Visit Spring Bioscience at https://www.springbio.com for more information and to purchase this product for your lab.